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MathWorks Inc cycle morlet wavelet
Cycle Morlet Wavelet, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc anti eif4e
EB2 interacts in vitro with the eIF4G and PABP initiation factors through its C-terminal domain. (A) Schematic representation of both recombinant GST-EB2 fusion proteins and the eIF4G plus eIF4G deletion mutants used. (B) GST pulldown assays were performed using GST, GST-EB2, GST-EB2Nter, or GST-EB2Cter and [35S]methionine-radiolabeled proteins eIF4GNter, eIF4Gp100, PABP, or <t>eIF4E,</t> synthesized in vitro in a rabbit reticulocyte lysate (RRL), under untreated or RNase-treated conditions as indicated. (C) GST pulldown assays were also performed with GST or GST-PABP fusion proteins and [35S]methionine-radiolabeled proteins EB2 (full length), EB2Nter, or EB2Cter, synthesized in vitro in RRL, under untreated or RNase-treated conditions. Eluates were subjected to SDS-PAGE and analyzed by autoradiography. In lane 1, the equivalent of 1/10 of the total RRL used in each assay was loaded onto the gel as an input control.
Anti Eif4e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti eif4e/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
anti eif4e - by Bioz Stars, 2026-06
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90
Altomed Limited morlet astigmatic ruler
EB2 interacts in vitro with the eIF4G and PABP initiation factors through its C-terminal domain. (A) Schematic representation of both recombinant GST-EB2 fusion proteins and the eIF4G plus eIF4G deletion mutants used. (B) GST pulldown assays were performed using GST, GST-EB2, GST-EB2Nter, or GST-EB2Cter and [35S]methionine-radiolabeled proteins eIF4GNter, eIF4Gp100, PABP, or <t>eIF4E,</t> synthesized in vitro in a rabbit reticulocyte lysate (RRL), under untreated or RNase-treated conditions as indicated. (C) GST pulldown assays were also performed with GST or GST-PABP fusion proteins and [35S]methionine-radiolabeled proteins EB2 (full length), EB2Nter, or EB2Cter, synthesized in vitro in RRL, under untreated or RNase-treated conditions. Eluates were subjected to SDS-PAGE and analyzed by autoradiography. In lane 1, the equivalent of 1/10 of the total RRL used in each assay was loaded onto the gel as an input control.
Morlet Astigmatic Ruler, supplied by Altomed Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/morlet astigmatic ruler/product/Altomed Limited
Average 90 stars, based on 1 article reviews
morlet astigmatic ruler - by Bioz Stars, 2026-06
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EB2 interacts in vitro with the eIF4G and PABP initiation factors through its C-terminal domain. (A) Schematic representation of both recombinant GST-EB2 fusion proteins and the eIF4G plus eIF4G deletion mutants used. (B) GST pulldown assays were performed using GST, GST-EB2, GST-EB2Nter, or GST-EB2Cter and [35S]methionine-radiolabeled proteins eIF4GNter, eIF4Gp100, PABP, or eIF4E, synthesized in vitro in a rabbit reticulocyte lysate (RRL), under untreated or RNase-treated conditions as indicated. (C) GST pulldown assays were also performed with GST or GST-PABP fusion proteins and [35S]methionine-radiolabeled proteins EB2 (full length), EB2Nter, or EB2Cter, synthesized in vitro in RRL, under untreated or RNase-treated conditions. Eluates were subjected to SDS-PAGE and analyzed by autoradiography. In lane 1, the equivalent of 1/10 of the total RRL used in each assay was loaded onto the gel as an input control.

Journal: Journal of Virology

Article Title: Epstein-Barr Virus Protein EB2 Stimulates Translation Initiation of mRNAs through Direct Interactions with both Poly(A)-Binding Protein and Eukaryotic Initiation Factor 4G

doi: 10.1128/JVI.01917-17

Figure Lengend Snippet: EB2 interacts in vitro with the eIF4G and PABP initiation factors through its C-terminal domain. (A) Schematic representation of both recombinant GST-EB2 fusion proteins and the eIF4G plus eIF4G deletion mutants used. (B) GST pulldown assays were performed using GST, GST-EB2, GST-EB2Nter, or GST-EB2Cter and [35S]methionine-radiolabeled proteins eIF4GNter, eIF4Gp100, PABP, or eIF4E, synthesized in vitro in a rabbit reticulocyte lysate (RRL), under untreated or RNase-treated conditions as indicated. (C) GST pulldown assays were also performed with GST or GST-PABP fusion proteins and [35S]methionine-radiolabeled proteins EB2 (full length), EB2Nter, or EB2Cter, synthesized in vitro in RRL, under untreated or RNase-treated conditions. Eluates were subjected to SDS-PAGE and analyzed by autoradiography. In lane 1, the equivalent of 1/10 of the total RRL used in each assay was loaded onto the gel as an input control.

Article Snippet: Immunopurified proteins were then analyzed by Western blotting using anti-Flag (Sigma-Aldrich), anti-PABPC1 (Sigma-Aldrich), anti-eIF4A (Cell Signaling Technology), anti-eIF4GI, anti-eIF4B, and anti-eIF4E (kindly provided by Simon Morley, University of Sussex, UK), anti-eIF2α (Cell Signaling Technology), anti-eIF3 (kindly provided by Pierre Jalinot, LBMC-ENS de Lyon, France), anti-rpS6 (Cell Signaling Technology), anti-BMRF1 (Cell Signaling Technology), anti-rpL11 (Cell Signaling Technology), anti-NXF1, and anti-CBP80 (kindly provided by Elisa Izaurralde, Max Planck Institute for Developmental Biology, Germany).

Techniques: In Vitro, Recombinant, Synthesized, SDS Page, Autoradiography